MethoCult™ GF M3534 is optimized for the growth and enumeration of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M) in colony-forming unit (CFU) assays of mouse bone marrow, spleen, peripheral blood, and fetal liver cells. MethoCult™ M3534 does not support the growth of erythroid progenitor cells (BFU-E and CFU-E) as it does not contain erythropoietin (EPO). This formulation is compatible with STEMvision™ software for automated colony counting of mouse bone marrow CFU assays.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/technical-resources/educational-materials/tech-tips/frequently-asked-questions-cfu-assay.html) on performing the CFU assay.